Bio Analytics
SHASTABIO offers various analytical chemistry services utilizing LC/MS, GC/MS, ICP/MS, and RT-PCR techniques. We provide drug analysis (pain management), food safety (Pesticide and Herbicide analysis), and Proteomics.
Small Molecule - cycle the drug discovery feedback loop
DMPK (drug metabolism and pharmacokinetics) and ADME (absorption, distribution, metabolism, and excretion)
- 1. Preclinical pharmacokinetic and toxicokinetic studies requiring bioanalytical quantitation using LC/MS/MS triple quadrupole tandem mass spectrometry
- 2. Preclinical drug metabolism studies requiring metabolite discovery and/or structural elucidation of metabolites in various biological matrices
Large Molecule – Oligonucleotide analysis, recombinant proteins & proteomics
Nano LC with Q-TOF (Time of Flight tandem mass analysis)
- Measurement of intact protein molecular ion by electrospray ionization mass spectrometry
- Protein characterization requiring peptide sequence coverage of proteolytic fragments, de novo sequencing, or post-translational modifications using Nanobore HPLC and quadrupole-time-of-flight tandem mass analysis
- Oligonucleotide characterization and analysis are important to drug development, registration, and quality control. Oligonucleotide therapeutics are short, single- or double-stranded DNA or RNA molecules that bind via Watson-Crick base pairing to enhance or repress the expression of target RNA, to treat or manage a wide range of diseases.
Biomarker Support
- For steroid, protein, and amino acid analysis (peptides) providing biomarker data to improve the effectiveness of drug development.
- ShastaBio provides high-quality, mass spectrometer based and immunochemical analysis for the development and measurement of biomarkers to improve the effectiveness of drug development. This includes targeted and non-targeted biochemical profiling.
- ShastaBio has accurate and reliable LCMS methods for peptide analysis. The methods have the precision and reproducibility criteria required by small and large molecule analysis.
Metabolite analysis
- The most abundant metabolites are identified by using mass measurements and Multiple Mass Defect Filter (MMDF) to Accurate Mass Data for Fast, Sensitive, and Accurate Identification of Metabolites.
- Metabolite identification using in vitro (hepatic microsomes and hepatocytes) or in vivo (plasma, bile) samples
- Polar Metabolite Analysis – small, hydrophilic polar metabolites such as amino acids, nucleic acids, sugars, and small organic acids, often involved in primary metabolism.
- Non-polar Metabolite Analysis – generally non-polar metabolites not directly involved in primary metabolism, such as antibiotics, polyketides, and phenolics.
Plasma protein binding study
- Drug to plasma-protein binding studies using rapid equilibrium dialysis (RED)
ICPMS, GCMS and RT PCR analysis
We are equipped with the necessary tools and expertise for additional service that you may need
- Real-Time PCR services for virus and microbial - Microbial impurities testing using RT PCR, Shiga toxin-producing Escherichia coli, Salmonella spp Pathogenic, Aspergillus species A. fumigatus, A. flavus, A. niger, A. terreus
- Metals and heavy metals analysis using ICPMS - Heavy Metal (Arsenic, Cadmium, Lead, Mercury) contamination testing using ICPMS
- Residual solvents and a small molecule analysis using Headspace gas chromatography/ mass spectroscopy. Residual solvents are 1,2-Dichloroethane, Benzene, Chloroform, Ethylene oxide, Methylene chloride, Trichloroethylene, Acetone, Acetonitrile, Butane, Ethanol, Ethyl acetate, Ethyl ether, Heptane, Hexane, Isopropyl alcohol, Methanol, Pentane, Propane, Toluene, Total xylenes (ortho-, meta-, para-)